Difference between revisions of "Methpipe"

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{| class="wikitable"
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|+ List of tools in MethPipe
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! Program !! Description
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|rmapbs, rmapbs-pe || map bisulfite treated short reads
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|-
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|duplicate-remover || remove duplicate reads
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|-
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|methcounts ||calculate methylation level and read coverage at individual sites
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|-
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|bsrate ||estimate bisulfite conversion rate
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|-
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|hmr ||identify hypo-methylated regions
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|-
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|hmr_plant || identify hyper-methylation in plants and organisms showing mosaic methylation
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|-
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|pmd || identify large partially methylated domains
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|-
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|amrfinder || identify allele-specific methylated regions
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|}
  
  

Revision as of 09:25, 12 September 2013

Application data

Created by Song Q et al
Biological application domain(s) Epigenomics, DNA methylation, Bisulfite Sequencing
Principal bioinformatics method(s) Bisulfite mapping, Analysis Pipeline
Technology Illumina
Created at University of Southern California
Maintained? Yes
Input format(s) FASTQ, SAM/BAM
Programming language(s) C++
Licence GPL (>= 3)
Operating system(s) Linux, Mac OS X
Contact: methpipe@googlegroups.com

Summary: The MethPipe software package is a computational pipeline for analyzing bisulfite sequencing data (BS-seq, WGBS and RRBS). MethPipe provides tools for mapping bisulfite sequencing read and estimating methylation levels at individual cytosine sites. Additionally, MethPipe includes tools for identifying higher-level methylation features, such as hypo-methylated regions (HMR), partially methylated domains (PMD), hyper-methylated regions (HyperMR), and allele-specific methylated regions (AMR).

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Description

List of tools in MethPipe
Program Description
rmapbs, rmapbs-pe map bisulfite treated short reads
duplicate-remover remove duplicate reads
methcounts calculate methylation level and read coverage at individual sites
bsrate estimate bisulfite conversion rate
hmr identify hypo-methylated regions
hmr_plant identify hyper-methylation in plants and organisms showing mosaic methylation
pmd identify large partially methylated domains
amrfinder identify allele-specific methylated regions






Links


References

  1. . 2013. PLoS One


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