Difference between revisions of "Cufflinks"

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|bio method=Assembly, Differentially expressed gene identification, statistical testing,
 
|bio method=Assembly, Differentially expressed gene identification, statistical testing,
 
|bio tech=Illumina, 454, Ion Torrent
 
|bio tech=Illumina, 454, Ion Torrent
|created at=University of Maryland, UC Berkeley, Caltech
+
|created by=Cole Trapnell
 +
|created at=University of Maryland, UC Berkeley, Johns Hopkins University,
 +
|maintained=Yes
 +
|email address=tophat.cufflinks@gmail.com
 
|input format=SAM,
 
|input format=SAM,
 
|output format=GTF
 
|output format=GTF

Revision as of 16:01, 16 September 2013

Application data

Created by Cole Trapnell
Biological application domain(s) RNA-Seq Alignment, RNA-Seq Quantitation, Differential Expression, Alternative Splicing, De novo transcriptome assembly, RNA-Seq
Principal bioinformatics method(s) Assembly, Differentially expressed gene identification, statistical testing
Technology Illumina, 454, Ion Torrent
Created at University of Maryland, UC Berkeley, Johns Hopkins University
Maintained? Yes
Input format(s) SAM
Output format(s) GTF
Licence Boost
Contact: tophat.cufflinks@gmail.com

Summary: Cufflinks assembles transcripts and estimates their abundances in RNA-Seq samples. It accepts aligned RNA-Seq reads and assembles the alignments into a parsimonious set of transcripts. Cufflinks then estimates the relative abundances of these transcripts based on how many reads support each one.

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Links


References

  1. . 2010. Nature Biotechnology


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