Methpipe

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Application data

Created by Song Q et al
Biological application domain(s) Epigenomics, DNA methylation, Bisulfite Sequencing
Principal bioinformatics method(s) Bisulfite mapping
Technology Illumina, Illumina HiSeq, Illumina Solexa
Created at University of Southern California
Maintained? Yes
Input format(s) FASTQ, SAM/BAM
Programming language(s) C++
Licence GPL (>= 3)
Operating system(s) Linux, Mac OS X
Contact: methpipe@googlegroups.com

Summary: The MethPipe software package is a computational pipeline for analyzing bisulfite sequencing data (BS-seq, WGBS and RRBS). MethPipe provides tools for mapping bisulfite sequencing read and estimating methylation levels at individual cytosine sites. Additionally, MethPipe includes tools for identifying higher-level methylation features, such as hypo-methylated regions (HMR), partially methylated domains (PMD), hyper-methylated regions (HyperMR), and allele-specific methylated regions (AMR).

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Description

List of tools in MethPipe

Program Description
rmapbs, rmapbs-pe map bisulfite treated short reads
duplicate-remover remove duplicate reads
methcounts calculate methylation level and read coverage at individual sites
bsrate estimate bisulfite conversion rate
hmr identify hypo-methylated regions
hmr_plant identify hyper-methylation in plants and organisms showing mosaic methylation
pmd identify large partially methylated domains
amrfinder identify allele-specific methylated regions


Links


References

  1. . 2013. PLoS One


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