19087247
This reference describes G-Mo.R-Seq.
PMID | PMID 19087247 |
---|---|
Title | Annotating genomes with massive-scale RNA sequencing. |
Year | 2008 |
Journal | Genome Biology |
Author | Denoeud F, Aury JM, Da Silva C, Noel B, Rogier O, Delledonne M, Morgante M, Valle G, Wincker P, Scarpelli C, Jaillon O, Artiguenave F. |
Volume | 9 |
Start page |
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Full text description
Next generation technologies enable massive-scale cDNA sequencing (so-called RNA-Seq). Mainly because of the difficulty of aligning short reads on exon-exon junctions, no attempts have been made so far to use RNA-Seq for building gene models de novo, that is, in the absence of a set of known genes and/or splicing events. We present G-Mo.R-Se (Gene Modelling using RNA-Seq), an approach aimed at building gene models directly from RNA-Seq and demonstrate its utility on the grapevine genome.