18264105
This reference describes Standalone hamming.
| PMID | PMID 18264105 |
|---|---|
| Title | Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex. |
| Year | 2008 |
| Journal | Nature Methods |
| Author | Hamady M, Walker JJ, Harris JK, Gold NJ, Knight R. |
| Volume | |
| Start page |
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Full text description
We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.
