19252504
This reference describes SNPSeeker.
PMID | PMID 19252504 |
---|---|
Title | Quantification of rare allelic variants from pooled genomic DNA. |
Year | 2009 |
Journal | Nature Methods |
Author | Druley TE, Vallania FL, Wegner DJ, Varley KE, Knowles OL, Bonds JA, Robison SW, Doniger SW, Hamvas A, Cole FS, Fay JC, Mitra RD. |
Volume | 6 |
Start page |
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Full text description
We report a targeted, cost-effective method to quantify rare single-nucleotide polymorphisms from pooled human genomic DNA using second-generation sequencing. We pooled DNA from 1,111 individuals and targeted four genes to identify rare germline variants. Our base-calling algorithm, SNPSeeker, derived from large deviation theory, detected single-nucleotide polymorphisms present at frequencies below the raw error rate of the sequencing platform.