20562415
This reference describes ICORN.
| PMID | PMID 20562415 |
|---|---|
| Title | Iterative Correction of Reference Nucleotides (iCORN) using second generation sequencing technology |
| Year | 2010 |
| Journal | Bioinformatics |
| Author | Otto TD, Sanders M, Berriman M, Newbold C |
| Volume | |
| Start page |
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Full text description
Motivation: The accuracy of reference genomes is important for downstream analysis but a low error rate requires expensive manual interrogation of the sequence. Here we describe a novel algorithm (iCORN) that iteratively aligns deep coverage of short sequencing reads to correct errors in reference genome sequences and evaluate their accuracy.
Results: Using Plasmodium falciparum (81 % AT content) as an extreme example we show that the algorithm is highly accurate and corrects over 2000 errors in the reference sequence. We give examples of its application to numerous other eukaryotic and prokaryotic genomes and suggest additional applications.
Availability: The software is available at http://icorn.sourceforge.net.