21109200
This reference describes CLCbio Genomics Workbench.
| PMID | PMID 21109200 |
|---|---|
| Title | The mtDNA mutation spectrum of the progeroid Polg mutator mouse includes abundant control region multimers. |
| Year | 2010 |
| Journal | Cell Metabolism |
| Author | Williams SL, Huang J, Edwards YJ, Ulloa RH, Dillon LM, Prolla TA, Vance JM, Moraes CT, Züchner S. |
| Volume | Dec 1;12(6) |
| Start page | 675 |
Error: No contents found at URL http://www.ebi.ac.uk/europepmc/webservices/rest/MED/21109200/citations/4000.
According to Europe PubMed Central, this reference has Error: no local variable "citations" was set. " Error: no local variable "citations" was set. " is not a number. citations.
For reference, you can check Google Scholar, which lacks an API because Google ...
Error: Invalid JSON. According to Almetric, this reference has an Altmetric score of Error: no local variable "altscore" was set. " Error: no local variable "altscore" was set. " is not a number..
Full text description
Polg mtDNA mutator mice are important models for investigating the role of acquired mtDNA mutations in aging. Despite extensive study, there remains little consensus on either the etiology of the progeroid phenotype or the mtDNA mutation spectrum induced by disrupted polymerase-γ function. To investigate the latter, we have developed a novel, pragmatic approach we term "Mito-seq," applying next-generation sequencing to enriched, native mtDNA. Regardless of detection parameters we observed an increase of at least two orders of magnitude in the number of mtDNA single nucleotide variants in Polg mutator mice compared to controls. We found no evidence for the accumulation of canonical mtDNA deletions but multimers of the mtDNA control region were identified in brain and heart. These control region multimers (CRMs) contained heterogeneous breakpoints and formed species that excluded the majority of mtDNA genes. CRMs demonstrate that polymerase-γ 3'-5' exonuclease activity is required for preserving mtDNA integrity.
