BSMAP
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Application data |
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| Created by | Yuanxin Xi |
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| Biological application domain(s) | DNA methylation |
| Principal bioinformatics method(s) | Read mapping, Bisulfite mapping |
| Created at | BCM |
| Maintained? | Maybe |
| Input format(s) | FASTA, FASTQ |
| Output format(s) | SOAP mapping format |
| Software features | Bisulfite sequencing |
| Programming language(s) | C++ |
| Licence | GPLv3 |
| Operating system(s) | Linux 64 |
Summary: short reads mapping software for bisulfite sequencing
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Bisulfite sequencing is a powerful technique to study DNA cytosine methylation. Bisulfite treatment followed by PCR amplification specifically converts unmethylated cytosines to thymine. Coupled with next generation sequencing technology, it is able to detect the methylation status of every cytosine in the genome. However, mapping high-throughput bisulfite reads to the reference genome remains a great challenge due to the increased searching space, reduced complexity of bisulfite sequence, asymmetric cytosine to thymine alignments, and multiple CpG heterogeneous methylation. We developed an efficient bisulfite reads mapping algorithm BSMAP to address the above issues. BSMAP combines genome hashing and bitwise masking to achieve fast and accurate bisulfite mapping. Compared with existing bisulfite mapping approaches, BSMAP is faster, more sensitive and more flexible. BSMAP is the first general-purpose bisulfite mapping software. It is able to map high-throughput bisulfite reads at whole genome level with feasible memory and CPU usage.
- based on SOAP
- read length up to 96 nt
- up to 5 mismatches
- support gapped mapping
- iterative trimming
- support parallel mapping
Links
References
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