MISO
Application data |
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Biological application domain(s) | RNA-Seq quantification, RNA-Seq, Alternative splicing |
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Technology | Any |
Maintained? | Yes |
Input format(s) | SAM/BAM |
Summary: An alternative to Cufflinks, MISO (Mixture-of-Isoforms) is a probabilistic framework that quantitates the expression level of alternatively spliced genes.
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Description
MISO (Mixture-of-Isoforms) is a probabilistic framework that quantitates the expression level of alternatively spliced genes from RNA-Seq data, and identifies differentially regulated isoforms or exons across samples. By modeling the generative process by which reads are produced from isoforms in RNA-Seq, the MISO model uses Bayesian inference to compute the probability that a read originated from a particular isoform.
Notes:
1) MISO requires all reads of equal length. i.e. Don't supply with quality trimmed reads.
Links
References
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