PIQA

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Application data

Principal bioinformatics method(s) Sequencing quality control
Technology Illumina
Maintained? Maybe
Input format(s) bustard, FASTQ, scarf
Programming language(s) R

Summary: PIQA is a quality analysis pipeline designed to examine genomic reads produced by Next Generation Sequencing technology (Illumina G1 Genome Analyzer). It is a set of libraries for R.

"Error: no local variable "counter" was set." is not a number.

Produces a bunch of report files and graphics describing run quality. The paper cited describes a base quality score range of -40 to 40, which doesn't seem to correspond to old/new Illumina pipelines or Sanger fastq quality scores. Note that PIQA will not process fastq files using Sanger quality scores.