ALLPATHS

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Application data

Biological application domain(s) Sequence assembly (de novo assembly)
Principal bioinformatics method(s) Sequence assembly, Sequence assembly (de-novo assembly)
Maintained? Yes
Input format(s) Fasta, Fastq

Summary: De novo assembly of whole-genome shotgun microreads.

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Previous versions of ALLPATHS are superseded by ALLPATH-LG. The latest version works on both small and large (mammalian size) genomes. To use it, you should first generate ~100 base Illumina reads from two libraries: one from ~180 bp fragments, and one from ~3000 bp fragments, both at about 45x coverage. Sequence from longer fragments will enable longer-range continuity.

Links


References

  1. . 2008. Genome Research
  2. . 2009. Genome Biology
  3. . 2010. PNAS


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