Ngs backbone

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Application data

Created by Blanca J, Ziarsolo P
Biological application domain(s) SNP detection, Genomics
Principal bioinformatics method(s) Read mapping, Sequence assembly
Technology Sanger, Illumina, 454
Created at COMAV at Universidad Politecnica de Valencia
Maintained? Yes
Input format(s) FASTA, FASTQ
Output format(s) BAM, VCF, GFF
Licence AGPL
Operating system(s) UNIX

Summary: ngs_backbone is a bioinformatic application created to work on sequence analysis by using NGS (Next Generation Sequencing) and sanger sequences. It is capable of cleaning reads, do de novo assembly or mapping against a reference and annotate SNPs, SSRs, ORFs, GO terms and sequence descriptions.

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ngs_backbone is a bioinformatic application created to work on sequence analysis by using NGS (Next Generation Sequencing) and sanger sequences. It is capable of cleaning reads, do de novo assembly or mapping against a reference and annotate SNPs, SSRs, ORFs, GO terms and sequence descriptions. Our laboratory is focused on transcriptomic analysis, so the tool has been used and tested on transcriptomes. Some analyses will be useful for genome analysis, but since our work deals mainly with transcriptomes design tradeoffs in ngs_backbone reflect this.

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