RDiff

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Application data

Created by Drewe P
Biological application domain(s) Alternative splicing, RNA-Seq, Transcriptomics
Principal bioinformatics method(s) Sequence alignment, Differential expression analysis
Technology Illumina HiSeq, Illumina Solexa, Illumina
Created at Memorial Sloan-Kettering Cancer Center
Maintained? Yes
Input format(s) SAM, BAM, GFF3
Output format(s) Tab-delimited file
Programming language(s) Python, Matlab
Licence Open Source
Operating system(s) Linux, Mac OS X

Summary: rDiff is an open source tool for accurate detection of differential RNA processing from RNA-Seq data. It implements two statistical tests to detect changes of the RNA processing between two samples. rDiff.parametric is a powerful test, which can be applied for well annotated organisms to detect changes in the relative abundance of isoforms. rDiff.nonparametric is an alternative when the annotation is incomplete or missing.

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Links


References

  1. . 2013. Nucleic Acids Research


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